Confocal Microscopy of Double Fertilization in Lily (Lilium)

Lily is one of the most frequently studied models of double fertilization. This site illustrates this process with some images taken from commercial slides using a confocal microscope. These are in stereopairs. Cross your eyes to see the three-dimensional effect.

First movie is a tilt-series*:
View the movie
Warning: 6.3MB file

Second series is thru-focus**:
View the movie
Warning: 10MB file

First movie is a tilt-series*:
View the movie
Warning: 18MB file

Second series is thru-focus**:
View the movie
Warning: 2.5MB file

*This is tilted in the computer and shows how the tissue appears if tilted from left to right. The screen capture is from this series.
**The thru-focus series shows a mixture of a Nomarski interference contrast image, with the fluorescence image. This is an orange on gray series showing each of the planes in order from top to bottom as viewed in the microscope.

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